Using barley seeds (Hordeum sativum Jess, var.), the influences of gibberellic acid (GA) and idole-3-acetic acid (IAA) on the amylase synthesis and that of the nucleic acid metabolism were investigated.
1. With the deembryonized barley seeds, the increase of amylase treated with a 10^-5M of GA and the decrease of amylase treated with 10^-5M IAA were matched by a proportionate increase and decrease in the amount of RNA. The influence of the hormones on the RNA synthesis has appeared immediately after the treatment but on the amylase synthesis it has appeared 8 hours later. But no influence on the DNA synthesis was observed on both hormones.
2. The amylase from deembryonized barley seeds treated with GA and IAA have been fractionated by gel fiteration on Sephadex G-100. The amylase components showed four fractions on both enzymes treated with GA and IAA. Fraction Ⅰ(FI) was differed from fraction Ⅳ (FIV) in Km value and the effects of temperature, pH and metal ions. On the basis of their emzymatic properties, it was considered that the FI was β-amylase and FIV was α-amylase. The influences of GA and IAA on each fractions appeared to be similar but on the amylase units per souble protein, IAA inhibited the production of amylaes FIV while it promoted that of amylase FI.
3. An experment was conducted to determine whether IAA inhibits GA-promoted amylase synthesis competitively or non-competitively. Using a Lineweaver-Burk plot, it was clear that IAA was acting in a non-competitive fachion. From this, IAA was probably not competing with GA at the same site, but it was acting at some other site which resulted in partial blocking of the action of GA on the amylase synthesis.
|